Construct: shRNA TRCN0000164435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030795.2-632s1c1
DNA Barcode:
CCCAGAAGATGGAATCCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STMN4 (81551)
Gene Description:
stathmin 4
Transcript:
RefSeq NM_030795.2 (NON-CURRENT)
Match location:
Position 632 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAGATGGAATCCAACA
Hairpin Sequence:
5'-CCGG-CCCAGAAGATGGAATCCAACA-CTCGAG-TGTTGGATTCCATCTTCTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAGATGGAATCCAACACTCGAGTGTTGGATTCCATCTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAGATGGAATCCAACACTCGAGTGTTGGATTCCATCTTCTGGG-3'

Other clones with same target sequence:

(none)