Construct: shRNA TRCN0000164445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152288.1-1033s1c1
DNA Barcode:
CAGGAACTAGAGGAACTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ORAI3 (93129)
Gene Description:
ORAI calcium release-activated calcium modulator 3
Transcript:
RefSeq NM_152288.1 (NON-CURRENT)
Match location:
Position 1033 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAACTAGAGGAACTGAAT
Hairpin Sequence:
5'-CCGG-CAGGAACTAGAGGAACTGAAT-CTCGAG-ATTCAGTTCCTCTAGTTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAACTAGAGGAACTGAATCTCGAGATTCAGTTCCTCTAGTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAACTAGAGGAACTGAATCTCGAGATTCAGTTCCTCTAGTTCCTG-3'

Other clones with same target sequence:

(none)