Construct: shRNA TRCN0000164567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004711.3-1462s1c1
DNA Barcode:
CCTGCTCAGTGTTACTGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNGR1 (9145)
Gene Description:
synaptogyrin 1
Transcript:
RefSeq NM_004711.3 (NON-CURRENT)
Match location:
Position 1462 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCAGTGTTACTGTCAT
Hairpin Sequence:
5'-CCGG-CCTGCTCAGTGTTACTGTCAT-CTCGAG-ATGACAGTAACACTGAGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCAGTGTTACTGTCATCTCGAGATGACAGTAACACTGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCAGTGTTACTGTCATCTCGAGATGACAGTAACACTGAGCAGG-3'

Other clones with same target sequence:

(none)