Construct: shRNA TRCN0000164576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017785.2-1812s1c1
DNA Barcode:
CCAGGTTAGCTGCTGAATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPDL1 (54908)
Gene Description:
spindle apparatus coiled-coil protein 1
Transcript:
RefSeq NM_017785.2 (NON-CURRENT)
Match location:
Position 1812 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGTTAGCTGCTGAATCAA
Hairpin Sequence:
5'-CCGG-CCAGGTTAGCTGCTGAATCAA-CTCGAG-TTGATTCAGCAGCTAACCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGTTAGCTGCTGAATCAACTCGAGTTGATTCAGCAGCTAACCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGTTAGCTGCTGAATCAACTCGAGTTGATTCAGCAGCTAACCTGG-3'

Other clones with same target sequence:

(none)