Construct: shRNA TRCN0000164640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002896.1-341s1c1
DNA Barcode:
GCCAAGTTTGAGGAGTATGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM4 (5936)
Gene Description:
RNA binding motif protein 4
Transcript:
RefSeq NM_002896.1 (NON-CURRENT)
Match location:
Position 341 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGTTTGAGGAGTATGGT
Hairpin Sequence:
5'-CCGG-GCCAAGTTTGAGGAGTATGGT-CTCGAG-ACCATACTCCTCAAACTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGTTTGAGGAGTATGGTCTCGAGACCATACTCCTCAAACTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGTTTGAGGAGTATGGTCTCGAGACCATACTCCTCAAACTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000338595