Construct: shRNA TRCN0000164729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207511.1-3261s1c1
DNA Barcode:
CCAAGGTGAAATCTGCCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf139 (401563)
Gene Description:
chromosome 9 open reading frame 139
Transcript:
RefSeq NM_207511.1 (NON-CURRENT)
Match location:
Position 3261 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGTGAAATCTGCCTCTT
Hairpin Sequence:
5'-CCGG-CCAAGGTGAAATCTGCCTCTT-CTCGAG-AAGAGGCAGATTTCACCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGTGAAATCTGCCTCTTCTCGAGAAGAGGCAGATTTCACCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGTGAAATCTGCCTCTTCTCGAGAAGAGGCAGATTTCACCTTGG-3'

Other clones with same target sequence:

(none)