Construct: shRNA TRCN0000164799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015926.3-871s1c1
DNA Barcode:
GAGACTTCTATGTGCCTGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEX264 (51368)
Gene Description:
testis expressed 264, ER-phagy receptor
Transcript:
RefSeq NM_015926.3 (NON-CURRENT)
Match location:
Position 871 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTTCTATGTGCCTGAGA
Hairpin Sequence:
5'-CCGG-GAGACTTCTATGTGCCTGAGA-CTCGAG-TCTCAGGCACATAGAAGTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTTCTATGTGCCTGAGACTCGAGTCTCAGGCACATAGAAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTTCTATGTGCCTGAGACTCGAGTCTCAGGCACATAGAAGTCTC-3'

Other clones with same target sequence:

(none)