Construct: shRNA TRCN0000164816

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001015071.1-2731s1c1
DNA Barcode:
CGGAGGTCACAAGTGAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC389281 (389281)
Gene Description:
similar to bA110H4.2 (similar to membrane protein)
Transcript:
RefSeq NM_001015071.1 (NON-CURRENT)
Match location:
Position 2731 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164816)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGTCACAAGTGAGATAT
Hairpin Sequence:
5'-CCGG-CGGAGGTCACAAGTGAGATAT-CTCGAG-ATATCTCACTTGTGACCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGTCACAAGTGAGATATCTCGAGATATCTCACTTGTGACCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGTCACAAGTGAGATATCTCGAGATATCTCACTTGTGACCTCCG-3'

Other clones with same target sequence:

(none)