Construct: shRNA TRCN0000164941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138401.1-331s1c1
DNA Barcode:
GCCTCTGTGTCCAAGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MVB12A (93343)
Gene Description:
multivesicular body subunit 12A
Transcript:
RefSeq NM_138401.1 (NON-CURRENT)
Match location:
Position 331 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTGTGTCCAAGAAGAAA
Hairpin Sequence:
5'-CCGG-GCCTCTGTGTCCAAGAAGAAA-CTCGAG-TTTCTTCTTGGACACAGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTGTGTCCAAGAAGAAACTCGAGTTTCTTCTTGGACACAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTGTGTCCAAGAAGAAACTCGAGTTTCTTCTTGGACACAGAGGC-3'

Other clones with same target sequence:

(none)