Construct: shRNA TRCN0000165014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032289.1-1813s1c1
DNA Barcode:
GCGTGGCTGGAAGAAATTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSD2 (84249)
Gene Description:
pleckstrin and Sec7 domain containing 2
Transcript:
RefSeq NM_032289.1 (NON-CURRENT)
Match location:
Position 1813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGGCTGGAAGAAATTCTA
Hairpin Sequence:
5'-CCGG-GCGTGGCTGGAAGAAATTCTA-CTCGAG-TAGAATTTCTTCCAGCCACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGGCTGGAAGAAATTCTACTCGAGTAGAATTTCTTCCAGCCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGGCTGGAAGAAATTCTACTCGAGTAGAATTTCTTCCAGCCACGC-3'

Other clones with same target sequence:

  1. TRCN0000110102