Construct: shRNA TRCN0000165058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002951.2-1002s1c1
DNA Barcode:
CGCGATCTTCAGCAAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPN2 (6185)
Gene Description:
ribophorin II
Transcript:
RefSeq NM_002951.2 (NON-CURRENT)
Match location:
Position 1002 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGATCTTCAGCAAGAAGAA
Hairpin Sequence:
5'-CCGG-CGCGATCTTCAGCAAGAAGAA-CTCGAG-TTCTTCTTGCTGAAGATCGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGATCTTCAGCAAGAAGAACTCGAGTTCTTCTTGCTGAAGATCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGATCTTCAGCAAGAAGAACTCGAGTTCTTCTTGCTGAAGATCGCG-3'

Other clones with same target sequence:

(none)