Construct: shRNA TRCN0000165143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178276.2-1419s1c1
DNA Barcode:
GCCATGTGGAACTCTGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERINC5 (256987)
Gene Description:
serine incorporator 5
Transcript:
RefSeq NM_178276.2 (NON-CURRENT)
Match location:
Position 1419 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTGGAACTCTGAGAAA
Hairpin Sequence:
5'-CCGG-GCCATGTGGAACTCTGAGAAA-CTCGAG-TTTCTCAGAGTTCCACATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTGGAACTCTGAGAAACTCGAGTTTCTCAGAGTTCCACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTGGAACTCTGAGAAACTCGAGTTTCTCAGAGTTCCACATGGC-3'

Other clones with same target sequence:

(none)