Construct: shRNA TRCN0000165174

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005096.2-3101s1c1
DNA Barcode:
GAGGCAGACTTCCCTAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMYM3 (9203)
Gene Description:
zinc finger MYM-type containing 3
Transcript:
RefSeq NM_005096.2 (NON-CURRENT)
Match location:
Position 3101 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165174)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCAGACTTCCCTAAGAAT
Hairpin Sequence:
5'-CCGG-GAGGCAGACTTCCCTAAGAAT-CTCGAG-ATTCTTAGGGAAGTCTGCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCAGACTTCCCTAAGAATCTCGAGATTCTTAGGGAAGTCTGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCAGACTTCCCTAAGAATCTCGAGATTCTTAGGGAAGTCTGCCTC-3'

Other clones with same target sequence:

(none)