Construct: shRNA TRCN0000165180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015085.3-781s1c1
DNA Barcode:
GCAGTGGGACTGAGATTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAP1GAP2 (23108)
Gene Description:
RAP1 GTPase activating protein 2
Transcript:
RefSeq NM_015085.3 (NON-CURRENT)
Match location:
Position 781 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGGGACTGAGATTCAAT
Hairpin Sequence:
5'-CCGG-GCAGTGGGACTGAGATTCAAT-CTCGAG-ATTGAATCTCAGTCCCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGGGACTGAGATTCAATCTCGAGATTGAATCTCAGTCCCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGGGACTGAGATTCAATCTCGAGATTGAATCTCAGTCCCACTGC-3'

Other clones with same target sequence:

(none)