Construct: shRNA TRCN0000165293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016210.2-789s1c1
DNA Barcode:
GAAGAAGAAGAGGCTGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C3orf18 (51161)
Gene Description:
chromosome 3 open reading frame 18
Transcript:
RefSeq NM_016210.2 (NON-CURRENT)
Match location:
Position 789 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAGAAGAGGCTGGAGAA
Hairpin Sequence:
5'-CCGG-GAAGAAGAAGAGGCTGGAGAA-CTCGAG-TTCTCCAGCCTCTTCTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAGAAGAGGCTGGAGAACTCGAGTTCTCCAGCCTCTTCTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAGAAGAGGCTGGAGAACTCGAGTTCTCCAGCCTCTTCTTCTTC-3'

Other clones with same target sequence:

(none)