Construct: shRNA TRCN0000165388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004564.1-1706s1c1
DNA Barcode:
CCCAAGGGACAACAACAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GATB (5188)
Gene Description:
glutamyl-tRNA amidotransferase subunit B
Transcript:
RefSeq NM_004564.1 (NON-CURRENT)
Match location:
Position 1706 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGGGACAACAACAAACA
Hairpin Sequence:
5'-CCGG-CCCAAGGGACAACAACAAACA-CTCGAG-TGTTTGTTGTTGTCCCTTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGGGACAACAACAAACACTCGAGTGTTTGTTGTTGTCCCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGGGACAACAACAAACACTCGAGTGTTTGTTGTTGTCCCTTGGG-3'

Other clones with same target sequence:

(none)