Construct: shRNA TRCN0000165428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018671.2-2353s1c1
DNA Barcode:
GCCCATGATAGAAGGCTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UNC45A (55898)
Gene Description:
unc-45 myosin chaperone A
Transcript:
RefSeq NM_018671.2 (NON-CURRENT)
Match location:
Position 2353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATGATAGAAGGCTACAT
Hairpin Sequence:
5'-CCGG-GCCCATGATAGAAGGCTACAT-CTCGAG-ATGTAGCCTTCTATCATGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATGATAGAAGGCTACATCTCGAGATGTAGCCTTCTATCATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATGATAGAAGGCTACATCTCGAGATGTAGCCTTCTATCATGGGC-3'

Other clones with same target sequence:

(none)