Construct: shRNA TRCN0000165441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002533.2-1484s1c1
DNA Barcode:
GCAGCAATGTGTGCAGTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NVL (4931)
Gene Description:
nuclear VCP like
Transcript:
RefSeq NM_002533.2 (NON-CURRENT)
Match location:
Position 1484 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAATGTGTGCAGTCAAT
Hairpin Sequence:
5'-CCGG-GCAGCAATGTGTGCAGTCAAT-CTCGAG-ATTGACTGCACACATTGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAATGTGTGCAGTCAATCTCGAGATTGACTGCACACATTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAATGTGTGCAGTCAATCTCGAGATTGACTGCACACATTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000276287