Construct: shRNA TRCN0000165444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005057.1-1453s1c1
DNA Barcode:
GCCACCTAAGAAGAAACCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBBP5 (5929)
Gene Description:
RB binding protein 5, histone lysine methyltransferase complex subunit
Transcript:
RefSeq NM_005057.1 (NON-CURRENT)
Match location:
Position 1453 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTAAGAAGAAACCCAA
Hairpin Sequence:
5'-CCGG-GCCACCTAAGAAGAAACCCAA-CTCGAG-TTGGGTTTCTTCTTAGGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTAAGAAGAAACCCAACTCGAGTTGGGTTTCTTCTTAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTAAGAAGAAACCCAACTCGAGTTGGGTTTCTTCTTAGGTGGC-3'

Other clones with same target sequence:

(none)