Construct: shRNA TRCN0000165479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052870.1-1639s1c1
DNA Barcode:
GAGCAGGTGATATGGAGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX18 (112574)
Gene Description:
sorting nexin 18
Transcript:
RefSeq NM_052870.1 (NON-CURRENT)
Match location:
Position 1639 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGGTGATATGGAGTGTA
Hairpin Sequence:
5'-CCGG-GAGCAGGTGATATGGAGTGTA-CTCGAG-TACACTCCATATCACCTGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGGTGATATGGAGTGTACTCGAGTACACTCCATATCACCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGGTGATATGGAGTGTACTCGAGTACACTCCATATCACCTGCTC-3'

Other clones with same target sequence:

(none)