Construct: shRNA TRCN0000165597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007551.1-661s1c1
DNA Barcode:
GAATACTTGTCCCTGGAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CT45A5 (441521)
Gene Description:
cancer/testis antigen family 45 member A5
Transcript:
RefSeq NM_001007551.1 (NON-CURRENT)
Match location:
Position 661 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATACTTGTCCCTGGAGGAT
Hairpin Sequence:
5'-CCGG-GAATACTTGTCCCTGGAGGAT-CTCGAG-ATCCTCCAGGGACAAGTATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATACTTGTCCCTGGAGGATCTCGAGATCCTCCAGGGACAAGTATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATACTTGTCCCTGGAGGATCTCGAGATCCTCCAGGGACAAGTATTC-3'

Other clones with same target sequence:

  1. TRCN0000140625