Construct: shRNA TRCN0000165691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004988.3-457s1c1
DNA Barcode:
CTCTTGTATCCTGGAGTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA1 (4100)
Gene Description:
MAGE family member A1
Transcript:
RefSeq NM_004988.3 (NON-CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTGTATCCTGGAGTCCTT
Hairpin Sequence:
5'-CCGG-CTCTTGTATCCTGGAGTCCTT-CTCGAG-AAGGACTCCAGGATACAAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTGTATCCTGGAGTCCTTCTCGAGAAGGACTCCAGGATACAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTGTATCCTGGAGTCCTTCTCGAGAAGGACTCCAGGATACAAGAG-3'

Other clones with same target sequence:

(none)