Construct: shRNA TRCN0000165718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003321.3-945s1c1
DNA Barcode:
CATAGCAAGAACATCCGCACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUFM (7284)
Gene Description:
Tu translation elongation factor, mitochondrial
Transcript:
RefSeq NM_003321.3 (NON-CURRENT)
Match location:
Position 945 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGCAAGAACATCCGCACT
Hairpin Sequence:
5'-CCGG-CATAGCAAGAACATCCGCACT-CTCGAG-AGTGCGGATGTTCTTGCTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGCAAGAACATCCGCACTCTCGAGAGTGCGGATGTTCTTGCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGCAAGAACATCCGCACTCTCGAGAGTGCGGATGTTCTTGCTATG-3'

Other clones with same target sequence:

  1. TRCN0000280936