Construct: shRNA TRCN0000165736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175901.3-1946s1c1
DNA Barcode:
CCTCCCTAAGTGTTGGGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL19-AS1 (283932)
Gene Description:
FBXL19 antisense RNA 1
Transcript:
RefSeq NM_175901.3 (NON-CURRENT)
Match location:
Position 1946 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCTAAGTGTTGGGATTA
Hairpin Sequence:
5'-CCGG-CCTCCCTAAGTGTTGGGATTA-CTCGAG-TAATCCCAACACTTAGGGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCTAAGTGTTGGGATTACTCGAGTAATCCCAACACTTAGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCTAAGTGTTGGGATTACTCGAGTAATCCCAACACTTAGGGAGG-3'

Other clones with same target sequence:

(none)