Construct: shRNA TRCN0000165746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010976.1-677s1c1
DNA Barcode:
CTCCCTGCTTTCTTTGTCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOL4L-DT (149950)
Gene Description:
NOL4L divergent transcript
Transcript:
RefSeq NM_001010976.1 (NON-CURRENT)
Match location:
Position 677 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCCTGCTTTCTTTGTCTGT
Hairpin Sequence:
5'-CCGG-CTCCCTGCTTTCTTTGTCTGT-CTCGAG-ACAGACAAAGAAAGCAGGGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCCTGCTTTCTTTGTCTGTCTCGAGACAGACAAAGAAAGCAGGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCCTGCTTTCTTTGTCTGTCTCGAGACAGACAAAGAAAGCAGGGAG-3'

Other clones with same target sequence:

(none)