Construct: shRNA TRCN0000165833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178514.3-1640s1c1
DNA Barcode:
GCTCACTACAACCTCCACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINC00346 (283487)
Gene Description:
long intergenic non-protein coding RNA 346
Transcript:
RefSeq NM_178514.3 (NON-CURRENT)
Match location:
Position 1640 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACTACAACCTCCACTTT
Hairpin Sequence:
5'-CCGG-GCTCACTACAACCTCCACTTT-CTCGAG-AAAGTGGAGGTTGTAGTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACTACAACCTCCACTTTCTCGAGAAAGTGGAGGTTGTAGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACTACAACCTCCACTTTCTCGAGAAAGTGGAGGTTGTAGTGAGC-3'

Other clones with same target sequence:

(none)