Construct: shRNA TRCN0000165903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203348.1-1201s1c1
DNA Barcode:
GACTTAGGAGACAGCGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC187 (399693)
Gene Description:
coiled-coil domain containing 187
Transcript:
RefSeq NM_203348.1 (NON-CURRENT)
Match location:
Position 1201 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTAGGAGACAGCGAGAAA
Hairpin Sequence:
5'-CCGG-GACTTAGGAGACAGCGAGAAA-CTCGAG-TTTCTCGCTGTCTCCTAAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTAGGAGACAGCGAGAAACTCGAGTTTCTCGCTGTCTCCTAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTAGGAGACAGCGAGAAACTCGAGTTTCTCGCTGTCTCCTAAGTC-3'

Other clones with same target sequence:

(none)