Construct: shRNA TRCN0000165954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005065.3-841s1c1
DNA Barcode:
GCCTCTGGACTTGGTGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEL1L (6400)
Gene Description:
SEL1L adaptor subunit of ERAD E3 ubiquitin ligase
Transcript:
RefSeq NM_005065.3 (NON-CURRENT)
Match location:
Position 841 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTGGACTTGGTGTTAAT
Hairpin Sequence:
5'-CCGG-GCCTCTGGACTTGGTGTTAAT-CTCGAG-ATTAACACCAAGTCCAGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTGGACTTGGTGTTAATCTCGAGATTAACACCAAGTCCAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTGGACTTGGTGTTAATCTCGAGATTAACACCAAGTCCAGAGGC-3'

Other clones with same target sequence:

(none)