Construct: shRNA TRCN0000165967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003665.1-3362s1c1
DNA Barcode:
GCAGAGAAAGTCAGAGGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STUM (375057)
Gene Description:
stum, mechanosensory transduction mediator homolog
Transcript:
RefSeq NM_001003665.1 (NON-CURRENT)
Match location:
Position 3362 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000165967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAAAGTCAGAGGCAAT
Hairpin Sequence:
5'-CCGG-GCAGAGAAAGTCAGAGGCAAT-CTCGAG-ATTGCCTCTGACTTTCTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAAAGTCAGAGGCAATCTCGAGATTGCCTCTGACTTTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAAAGTCAGAGGCAATCTCGAGATTGCCTCTGACTTTCTCTGC-3'

Other clones with same target sequence:

(none)