Construct: shRNA TRCN0000166145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198394.1-266s1c1
DNA Barcode:
CTCAGAAGCACCAGAATACGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf85 (138241)
Gene Description:
chromosome 9 open reading frame 85
Transcript:
RefSeq NM_198394.1 (NON-CURRENT)
Match location:
Position 266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGAAGCACCAGAATACGT
Hairpin Sequence:
5'-CCGG-CTCAGAAGCACCAGAATACGT-CTCGAG-ACGTATTCTGGTGCTTCTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGAAGCACCAGAATACGTCTCGAGACGTATTCTGGTGCTTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGAAGCACCAGAATACGTCTCGAGACGTATTCTGGTGCTTCTGAG-3'

Other clones with same target sequence:

(none)