Construct: shRNA TRCN0000166184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020145.2-914s1c1
DNA Barcode:
CGTCAAGTCTCAGACAACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3GLB2 (56904)
Gene Description:
SH3 domain containing GRB2 like, endophilin B2
Transcript:
RefSeq NM_020145.2 (NON-CURRENT)
Match location:
Position 914 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAAGTCTCAGACAACCTA
Hairpin Sequence:
5'-CCGG-CGTCAAGTCTCAGACAACCTA-CTCGAG-TAGGTTGTCTGAGACTTGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAAGTCTCAGACAACCTACTCGAGTAGGTTGTCTGAGACTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAAGTCTCAGACAACCTACTCGAGTAGGTTGTCTGAGACTTGACG-3'

Other clones with same target sequence:

(none)