Construct: shRNA TRCN0000166234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153215.1-436s1c1
DNA Barcode:
CAGGAGGAGACACTACTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSMEM2 (132228)
Gene Description:
leucine rich single-pass membrane protein 2
Transcript:
RefSeq NM_153215.1 (NON-CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGGAGACACTACTCAAA
Hairpin Sequence:
5'-CCGG-CAGGAGGAGACACTACTCAAA-CTCGAG-TTTGAGTAGTGTCTCCTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGGAGACACTACTCAAACTCGAGTTTGAGTAGTGTCTCCTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGGAGACACTACTCAAACTCGAGTTTGAGTAGTGTCTCCTCCTG-3'

Other clones with same target sequence:

(none)