Construct: shRNA TRCN0000166310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015462.2-207s1c1
DNA Barcode:
CCAGCTGTGTGCAACTTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOL11 (25926)
Gene Description:
nucleolar protein 11
Transcript:
RefSeq NM_015462.2 (NON-CURRENT)
Match location:
Position 207 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTGTGTGCAACTTTCAA
Hairpin Sequence:
5'-CCGG-CCAGCTGTGTGCAACTTTCAA-CTCGAG-TTGAAAGTTGCACACAGCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTGTGTGCAACTTTCAACTCGAGTTGAAAGTTGCACACAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTGTGTGCAACTTTCAACTCGAGTTGAAAGTTGCACACAGCTGG-3'

Other clones with same target sequence:

(none)