Construct: shRNA TRCN0000166352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032137.2-2808s1c1
DNA Barcode:
CTTCCGGTCTCAACAGGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C3orf20 (84077)
Gene Description:
chromosome 3 open reading frame 20
Transcript:
RefSeq NM_032137.2 (NON-CURRENT)
Match location:
Position 2808 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCGGTCTCAACAGGATTA
Hairpin Sequence:
5'-CCGG-CTTCCGGTCTCAACAGGATTA-CTCGAG-TAATCCTGTTGAGACCGGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCGGTCTCAACAGGATTACTCGAGTAATCCTGTTGAGACCGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCGGTCTCAACAGGATTACTCGAGTAATCCTGTTGAGACCGGAAG-3'

Other clones with same target sequence:

(none)