Construct: shRNA TRCN0000166355
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_052907.1-1099s1c1
- DNA Barcode:
- CCAAGGAGGCAGCAATGATAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TMEM132B (114795)
- Gene Description:
- transmembrane protein 132B
- Transcript:
- RefSeq NM_052907.1 (NON-CURRENT)
- Match location:
- Position 1099 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000166355)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAAGGAGGCAGCAATGATAT
- Hairpin Sequence:
-
5'-CCGG-CCAAGGAGGCAGCAATGATAT-CTCGAG-ATATCATTGCTGCCTCCTTGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAAGGAGGCAGCAATGATATCTCGAGATATCATTGCTGCCTCCTTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAAGGAGGCAGCAATGATATCTCGAGATATCATTGCTGCCTCCTTGG-3'
Other clones with same target sequence:
(none)
