Construct: shRNA TRCN0000166392
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020431.1-3154s1c1
- DNA Barcode:
- CGTAGACCTGAGAAGAGGTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TMEM63C (57156)
- Gene Description:
- transmembrane protein 63C
- Transcript:
- RefSeq NM_020431.1 (NON-CURRENT)
- Match location:
- Position 3154 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000166392)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGTAGACCTGAGAAGAGGTAA
- Hairpin Sequence:
-
5'-CCGG-CGTAGACCTGAGAAGAGGTAA-CTCGAG-TTACCTCTTCTCAGGTCTACG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGTAGACCTGAGAAGAGGTAACTCGAGTTACCTCTTCTCAGGTCTACGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGTAGACCTGAGAAGAGGTAACTCGAGTTACCTCTTCTCAGGTCTACG-3'
Other clones with same target sequence:
(none)
