Construct: shRNA TRCN0000166449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174891.2-760s1c1
DNA Barcode:
CTTCTGTCTCCAGCATTGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLBA1 (122616)
Gene Description:
clathrin binding box of aftiphilin containing 1
Transcript:
RefSeq NM_174891.2 (NON-CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTGTCTCCAGCATTGCAA
Hairpin Sequence:
5'-CCGG-CTTCTGTCTCCAGCATTGCAA-CTCGAG-TTGCAATGCTGGAGACAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTGTCTCCAGCATTGCAACTCGAGTTGCAATGCTGGAGACAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTGTCTCCAGCATTGCAACTCGAGTTGCAATGCTGGAGACAGAAG-3'

Other clones with same target sequence:

(none)