Construct: shRNA TRCN0000166452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207354.1-616s1c1
DNA Barcode:
CGTTGAGATGAAGTGGGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD13D (338692)
Gene Description:
ankyrin repeat domain 13D
Transcript:
RefSeq NM_207354.1 (NON-CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGAGATGAAGTGGGAGTT
Hairpin Sequence:
5'-CCGG-CGTTGAGATGAAGTGGGAGTT-CTCGAG-AACTCCCACTTCATCTCAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGAGATGAAGTGGGAGTTCTCGAGAACTCCCACTTCATCTCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGAGATGAAGTGGGAGTTCTCGAGAACTCCCACTTCATCTCAACG-3'

Other clones with same target sequence:

(none)