Construct: shRNA TRCN0000166502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033626.2-2658s1c1
DNA Barcode:
CCCTTCTGATATGTCCCTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC120 (90060)
Gene Description:
coiled-coil domain containing 120
Transcript:
RefSeq NM_033626.2 (NON-CURRENT)
Match location:
Position 2658 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCTGATATGTCCCTTGT
Hairpin Sequence:
5'-CCGG-CCCTTCTGATATGTCCCTTGT-CTCGAG-ACAAGGGACATATCAGAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCTGATATGTCCCTTGTCTCGAGACAAGGGACATATCAGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCTGATATGTCCCTTGTCTCGAGACAAGGGACATATCAGAAGGG-3'

Other clones with same target sequence:

(none)