Construct: shRNA TRCN0000166670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017928.1-519s1c1
DNA Barcode:
CTGAGGCTGTAGAAAGTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD40CL (55018)
Gene Description:
ANKRD40 C-terminal like
Transcript:
RefSeq NM_017928.1 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGGCTGTAGAAAGTGCTT
Hairpin Sequence:
5'-CCGG-CTGAGGCTGTAGAAAGTGCTT-CTCGAG-AAGCACTTTCTACAGCCTCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGGCTGTAGAAAGTGCTTCTCGAGAAGCACTTTCTACAGCCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGGCTGTAGAAAGTGCTTCTCGAGAAGCACTTTCTACAGCCTCAG-3'

Other clones with same target sequence:

(none)