Construct: shRNA TRCN0000166685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177964.3-998s1c1
DNA Barcode:
CTGGGTCTTTGTGCTTCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYPD6B (130576)
Gene Description:
LY6/PLAUR domain containing 6B
Transcript:
RefSeq NM_177964.3 (NON-CURRENT)
Match location:
Position 998 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGGTCTTTGTGCTTCCATT
Hairpin Sequence:
5'-CCGG-CTGGGTCTTTGTGCTTCCATT-CTCGAG-AATGGAAGCACAAAGACCCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGGTCTTTGTGCTTCCATTCTCGAGAATGGAAGCACAAAGACCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGGTCTTTGTGCTTCCATTCTCGAGAATGGAAGCACAAAGACCCAG-3'

Other clones with same target sequence:

(none)