Construct: shRNA TRCN0000166744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152363.2-631s1c1
DNA Barcode:
CCCTTGAAACTGTGGACAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKLE1 (126549)
Gene Description:
ankyrin repeat and LEM domain containing 1
Transcript:
RefSeq NM_152363.2 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGAAACTGTGGACAAAC
Hairpin Sequence:
5'-CCGG-CCCTTGAAACTGTGGACAAAC-CTCGAG-GTTTGTCCACAGTTTCAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGAAACTGTGGACAAACCTCGAGGTTTGTCCACAGTTTCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGAAACTGTGGACAAACCTCGAGGTTTGTCCACAGTTTCAAGGG-3'

Other clones with same target sequence:

(none)