Construct: shRNA TRCN0000166794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206910.1-1232s1c1
DNA Barcode:
CCTCAGGCTGTTTCTGCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUSBP4 (375513)
Gene Description:
GUSB pseudogene 4
Transcript:
RefSeq NM_206910.1 (NON-CURRENT)
Match location:
Position 1232 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGGCTGTTTCTGCAATT
Hairpin Sequence:
5'-CCGG-CCTCAGGCTGTTTCTGCAATT-CTCGAG-AATTGCAGAAACAGCCTGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGGCTGTTTCTGCAATTCTCGAGAATTGCAGAAACAGCCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGGCTGTTTCTGCAATTCTCGAGAATTGCAGAAACAGCCTGAGG-3'

Other clones with same target sequence:

(none)