Construct: shRNA TRCN0000166807

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014153.2-3312s1c1
DNA Barcode:
CAGATGGTGAAGTGACTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC3H7A (29066)
Gene Description:
zinc finger CCCH-type containing 7A
Transcript:
RefSeq NM_014153.2 (NON-CURRENT)
Match location:
Position 3312 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166807)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGGTGAAGTGACTGCTA
Hairpin Sequence:
5'-CCGG-CAGATGGTGAAGTGACTGCTA-CTCGAG-TAGCAGTCACTTCACCATCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGGTGAAGTGACTGCTACTCGAGTAGCAGTCACTTCACCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGGTGAAGTGACTGCTACTCGAGTAGCAGTCACTTCACCATCTG-3'

Other clones with same target sequence:

(none)