Construct: shRNA TRCN0000166849

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020854.2-3096s1c1
DNA Barcode:
CAAAGATATTTGCACTGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RELCH (57614)
Gene Description:
RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing
Transcript:
RefSeq NM_020854.2 (NON-CURRENT)
Match location:
Position 3096 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166849)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGATATTTGCACTGCTTT
Hairpin Sequence:
5'-CCGG-CAAAGATATTTGCACTGCTTT-CTCGAG-AAAGCAGTGCAAATATCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGATATTTGCACTGCTTTCTCGAGAAAGCAGTGCAAATATCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGATATTTGCACTGCTTTCTCGAGAAAGCAGTGCAAATATCTTTG-3'

Other clones with same target sequence:

(none)