Construct: shRNA TRCN0000166889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080862.1-1502s1c1
DNA Barcode:
CCTCTGAATAATGAATGATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPSB4 (92369)
Gene Description:
splA/ryanodine receptor domain and SOCS box containing 4
Transcript:
RefSeq NM_080862.1 (NON-CURRENT)
Match location:
Position 1502 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGAATAATGAATGATGA
Hairpin Sequence:
5'-CCGG-CCTCTGAATAATGAATGATGA-CTCGAG-TCATCATTCATTATTCAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGAATAATGAATGATGACTCGAGTCATCATTCATTATTCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGAATAATGAATGATGACTCGAGTCATCATTCATTATTCAGAGG-3'

Other clones with same target sequence:

(none)