Construct: shRNA TRCN0000166910

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032165.2-1949s1c1
DNA Barcode:
CACTCTCACTAACATCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRIQ1 (84125)
Gene Description:
leucine rich repeats and IQ motif containing 1
Transcript:
RefSeq NM_032165.2 (NON-CURRENT)
Match location:
Position 1949 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166910)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTCTCACTAACATCAGAAA
Hairpin Sequence:
5'-CCGG-CACTCTCACTAACATCAGAAA-CTCGAG-TTTCTGATGTTAGTGAGAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCTCACTAACATCAGAAACTCGAGTTTCTGATGTTAGTGAGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCTCACTAACATCAGAAACTCGAGTTTCTGATGTTAGTGAGAGTG-3'

Other clones with same target sequence:

(none)