Construct: shRNA TRCN0000166977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012506.1-2925s1c1
DNA Barcode:
CCTTCACATTTGATTTGTGTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC66 (285331)
Gene Description:
coiled-coil domain containing 66
Transcript:
RefSeq NM_001012506.1 (NON-CURRENT)
Match location:
Position 2925 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000166977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCACATTTGATTTGTGTC
Hairpin Sequence:
5'-CCGG-CCTTCACATTTGATTTGTGTC-CTCGAG-GACACAAATCAAATGTGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCACATTTGATTTGTGTCCTCGAGGACACAAATCAAATGTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCACATTTGATTTGTGTCCTCGAGGACACAAATCAAATGTGAAGG-3'

Other clones with same target sequence:

(none)