Construct: shRNA TRCN0000167001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018128.4-2550s1c1
DNA Barcode:
GAACTTTACTAACACTAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSR1 (55720)
Gene Description:
TSR1 ribosome maturation factor
Transcript:
RefSeq NM_018128.4 (NON-CURRENT)
Match location:
Position 2550 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTTTACTAACACTAGGAA
Hairpin Sequence:
5'-CCGG-GAACTTTACTAACACTAGGAA-CTCGAG-TTCCTAGTGTTAGTAAAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTTTACTAACACTAGGAACTCGAGTTCCTAGTGTTAGTAAAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTTTACTAACACTAGGAACTCGAGTTCCTAGTGTTAGTAAAGTTC-3'

Other clones with same target sequence:

(none)