Construct: shRNA TRCN0000167027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032270.2-773s1c1
DNA Barcode:
CTGAGAAGTTTGTAGTTGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC8C (84230)
Gene Description:
leucine rich repeat containing 8 VRAC subunit C
Transcript:
RefSeq NM_032270.2 (NON-CURRENT)
Match location:
Position 773 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGAAGTTTGTAGTTGATA
Hairpin Sequence:
5'-CCGG-CTGAGAAGTTTGTAGTTGATA-CTCGAG-TATCAACTACAAACTTCTCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGAAGTTTGTAGTTGATACTCGAGTATCAACTACAAACTTCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGAAGTTTGTAGTTGATACTCGAGTATCAACTACAAACTTCTCAG-3'

Other clones with same target sequence:

(none)